IMAGE

Fig. 2

ID
ZDB-IMAGE-130807-19
Genes
Antibodies
Source
Figures for Hochgreb-Hägele et al., 2013
Image
Figure Caption

Fig. 2 Mutation in a splice donor (SD) site of the lamb1a gene results in a truncated protein in s804 mutants and impairs expression of the multimeric protein laminin 1 in the basement membrane of lamb1as804 mutants. (A) The lamb1a ORF in zebrafish (exons are numbered). Functional domains of the corresponding protein are represented by colored boxes. Gray blocks represent the cloning strategy. (B) G-to-T replacement in the SD site for exon 24 of lamb1a in s804 mutants (6/6). (C) Alternative splicing in lamb1as804 mutants. Mutation of the SD site for exon 24 results in excision of exon 24 (i), maintenance of intron 24 (I24, ii) or splicing of exon 23 into a cryptic site within exon 24 (iii). These alternative splice events result in a frame-shift and premature stop signals (asterisks) that disrupt the C-terminal coiled-coil domains (blue boxes). (D) PCR amplification of block C from wild-type cDNA produces a single band of 1.5 kb. In s804 mutants, alternative products are detected. (E) Injection of a lamb1a splice-blocking MO recapitulates the s804 mutant phenotype. Scale bars: 20 μm. (F-H′) Transverse sections of wild-type (F,G,H) and lamb1as804 mutant (F′,G′,H′) embryos at 16 ss (F-G′) and 30 hpf (H,H2). Anterior (F,F′) and posterior (G,G′) level sections. Lam1 (red), Tg(XIEef1a:GFP) (green). L, left; R, right. Scale bars: 20 μm. Neural tube (nt, broken line), notochord (n), somite (s) and gut endoderm (e). Dotted line identifies marked atrophy of the notochord.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development