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Fig. S3 Analysis of MCC gene expression profile and multiciliogenesis. (A) Work flow of microarray analysis. (B) Expression analysis of upregulated ciliary genes in the microarray data by WISH. (C,D) The ciliary morphology (anti-acetylated tubulin staining, red, arrows) of MCCs (anti-GFP staining, green) at 30 hpf (C), 2 dpf and 3 dpf (D) in normal embryos and miR-34b morphants. (E) The ciliary morphology (red) at proximal and distal pronephric ducts of 36-hpf normal embryos and miR-34b morphants.
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