IMAGE

Fig. 7

ID
ZDB-IMAGE-130801-23
Genes
Source
Figures for Nichols et al., 2013
Image
Figure Caption

Fig. 7 barx1 negatively regulates hand2. (A-F′′) Wild-type and barx1 mutant animals were subjected to fluorescent in situ hybridization to reveal sox9a, nkx3.2 and hand2 expression during the pharyngula (A-B′′) and hatching (C-F′′) periods. Arrow indicates ventrally restricted hand2; arrowhead indicates expanded hand2 expression. All panels are single confocal sections. (G,H) Animals were subjected to in situ hybridization to reveal midline-restricted hand2 expression in wild type (arrow) and expanded hand2 expression in barx1 mutants (arrowhead). (I,J) hand2-expressing cells were transgenically labeled with E2Crimson (hand2:Crimson, red) and chondrocytes with EGFP (sox9a:EGFP, green), and imaged by time-lapse microscopy. Movie excerpts are z-projections from the ventral perspective. Arrow indicates downregulated hand2 reporter in differentiated cartilages; arrowhead indicates persistent hand2 reporter. Dorsal is upwards and anterior is towards the left in A-D; anterior is towards the top, while right is towards the left in E-J. Scale bars: 100 μm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development