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Fig. S1 The six1a morpholino targets the six1a exon1/intron1 splice junction. (A) Complementary sequence of six1a cDNA of control embryos and embryos injected with six1a morpholino, dashed line indicates premature splice site. (B) Control six1a cDNA and cDNA from six1a morpholino injected embryos run on an agarose gel shows a smaller product in the morphant embryos. (C) Brightfield (left) and fluorescent (right) pictures showing six1b:GFP embryos uninjected and injected with six1a ATG morpholino, illustrating the efficiency of the morpholino.
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Full text @ J. Cell Sci.