ZFIN Image: Taibi et al., 2013, Fig. 3

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Figure Caption

Fig. 3 Loss of chch does not significantly alter spatial distribution of mesodermal markers during gastrulation. A–J: Whole-mount RNA in situ hybridization of early (6 hours postfertilization [hpf], A–F) and late (9 hpf, G–I) gastrula stage zebrafish embryos (A–F) using probes for mesodermal markers (as indicated). Expression of no-tail (ntl), chordin (chd), and floating-head (flh) are comparable in chch mutants and wild-type embryos at 6 hpf. Likewise expression of myoD and foxa2 (axial) are not altered chch^sbu50/sbu50 mutants. Embryos were generated from a cross of a homozygous chch^sbu50/sbu50 female to a heterozygous male. Genotypes of embryos were established by polymerase chain reaction following photography.

Acknowledgments

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