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Fig. S4 Short-term NICD overexpression has no effect on blastema proliferation or on fin patterning. (A) NICD overexpression in hs:Gal4; UAS:NICD double-transgenic fish for 24 hours starting at 3 dpa does not induce cell proliferation, as revealed by BrdUincorporation. n= 6 fish (control), 22 blastemas; n= 8 fish (gain of function), 29 blastemas, n.s., not significant, Student’s t-test. (B) NICD overexpression in hs:Gal4; UAS:NICD double transgenic fish for 6 days starting immediately after amputation does not expand the distal-most blastema marked by anti-Aldh1a immunofluorescence detected in confocal images. n= 6/6 (control), n=5/6 (gain of function). (C) NICD overexpression in hs:Gal4; UAS:NICD double transgenic fish for 12 hours starting at 3 dpa does not alter lef1 or runx2b expression. n=7 fins (all groups).