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Fig. S3

ID
ZDB-IMAGE-130618-37
Source
Figures for Yao et al., 2013
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Figure Caption

Fig. S3 In situ hybridization to detect alk overexpression in alk:HSE:cfp transgenic embryos. A heat shock was performed at 24 hpf. All in situ hybridizations were done using an alk antisense RNA probe, at 1 hour post heat shock (hph) (A), 6 hph (B), 22 hph (C), and shortly after a second HS performed at 48 hpf (D). Inserts show representative transgenic positive embryos. As soon as 1 hour after heat shock, high levels of exogenous alk RNA could be detected in the entire embryo. Reduced staining at 6 hours after HS indicated RNA degradation. Staining almost completely disappeared at 22 hours after HS. A second HS re-activated transcription of overexpressed alk. Arrows points to transgene negative siblings where no exogenous alk was detected. Endogenous alk expression was not detected because of short staining time.

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