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Fig. 4 Effects of incubation time and oPRL on ncc (A), nhe3b (B), ecac (C), prlra (D), prlrb (E) and β-actin (F) gene expression in cultured gill filaments. Solid line, control; dashed line, oPRL (1.0 μg/ml). Gene expression is presented as fold-change from 0 h. Means ± SEM (n = 6). Filaments were dissected from anesthetized fish and pre-incubated for 3 h prior to treatment with oPRL-supplemented medium. Effects of hormone treatment and time were evaluated by two-way ANOVA. For clarity, only the results of follow-up analyses for a significant main effect of hormone treatment are presented. *, **, ***Significantly different from the time-matched controls at P < 0.05, 0.01 and 0.001, respectively, by Student’s t-test. Whole-mount in situ hybridization showing ncc expression in gill filaments cultured for 4 h in the presence or absence of oPRL as compared with pre-incubated and 0 h control filaments (G). Scale bar = 50 μm.
Reprinted from Molecular and Cellular Endocrinology, 369(1-2), Breves, J.P., Serizier, S.B., Goffin, V., McCormick, S.D., and Karlstrom, R.O., Prolactin regulates transcription of the ion uptake Na+/Cl- cotransporter (ncc) gene in zebrafish gill, 98-106, Copyright (2013) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.