Fig. 3

Fig. 3 Oocyte ablation in adult zebrafish induces complete ovary-to-testis reprogramming. (A-F) in situ hybridization of wild-type (WT) and sex-reverted gonads for genes differentially expressed in the male or female somatic gonad. (A) amh is not detected in WT ovaries. (B) cyp19a1a is expressed in granulosa cells. (C) amh is expressed in Sertoli cells of the testis while cyp19a1a (D) is not detected. (E) amh but not cyp19a1a (F) is expressed in testes of sex-reverted Tg(ziwi:CFP-NTR) females. (G-O) expression of CFP-NTR in control ovary, control testis, and testis of sex-reverted fish. (G, J, and M) merged images: CFP-NTR in green, DNA in blue. (H, K, and N) CFP-NTR only. (I, L, and O) DNA only. (G-I) CFP-NTR is expressed in all germ cells of the control ovary. IA^z, IA^p, IA^d, stage IA oocytes -- zygotene, pachytene, diplotene stages of meiotic prophase, IB, stage IB oocytes. Premeiotic germ cells (boxed and inset). (J-L) CFP-NTR expression in testis tissue from sex-reverted fish. (M-O) CFP-NTR expression in control testis. Morphology of control ovary (P), control testis (Q), and testis of sex-reverted fish (R). Sg, spermatogonia; Sc, spermatocytes; Sz, spermatozoa; S, Sertoli cells; L, Leydig cells; My, peritubular myoid cells. Scale bars: 200 μM (A-F, and P), 50 μM (G-I, Q, and R), 10 μM (G-I, insets), and 20 μM (J-O). See also Fig. S3.