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Fig. S18 Otpa and Sim1a are sufficient to induce supernumerary VIP neurons and Fezf2 and Foxb1.2 are sufficient to induce supernumerary Uts1 neurons. After an overnight heat shock, 2-dpf and 3-dpf wild-type embryos (A,G) and embryos containing Tg(hsp70:otpa-2A-EGFP)hd7 (B), Tg(hsp70:sim1a-2A-lyntdTomato)hd8 (C,H), Tg(hsp70:otpa-2A-EGFP)hd7; Tg(hsp70:sim1a- 2A-lyntdTomato)hd8 (D), Tg(hsp70:foxb1.2-2A-EGFP)hd9 (E,K), Tg(hsp70:fezf2-2A-EGFP)hd6 (F,I) and Tg(hsp70:sim1a-2AlyntdTomato) hd8; Tg(hsp70:fezf2-2A-EGFP)hd6 (J) were subjected to WISH to detect the expression of vip and uts1. (A-F) The overexpression of otpa (B; n=62/100), sim1a (C; n=75/129) and the combined overexpression of both (D; n=22/31) leads to supernumerary vip-expressing cells, whereas the overexpression of foxb1.2 (E; n=79/93) results in fewer vip-expressing cells and overexpression of fezf2 has no effect (F; n=105/105). (G-K) The overexpression of sim1a (H; n=152/152) results in no changes in uts1 cell number, whereas the overexpression of fezf2 (I; n=87/158), a combination of sim1a and fezf2 (J; n=25/47) and foxb1.2 (K; n=95/129) results in supernumerary uts1-expressing cells. Magnified dorsal views are shown. OE, overexpression. Scale bar: 50 μm.