|
Fig. S8 Rab11a MO knockdown disrupted KV development and left-right asymmetry. (A,B) KV cilia immunostained with acetylated tubulin antibodies showing a reduced KV size concomitant with a slight reduction in cilia number and length in embryos injected with an optimal dose of Rab11a MO (B) relative to MO control (A). The dashed circle outlines the approximate boundary of KV lumen. Scale bars: 20 μm. (C) Approximately 43% of the Rab11a MO embryos contained a reduced KV compared with MO control embryos. (D) Quantification of KV lumen area. (E,F) KV cilia number (E) and length (F) were significantly reduced in Rab11a MO embryos relative to controls. Error bars represent s.d. n, number of embryos analyzed. *P<0.05. (G) Consistent with KV defects, embryos injected with an optimal dose of Rab11a MO showed defects in heart left-right asymmetry. These defects were partially rescued by co-injecting rab11a mRNA. n, number of embryos analyzed.