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Fig. S3 Transcriptome analysis of cardiac gene expression upon Bmp up-regulation. (A) Enrichment analysis of genes up-regulated in Tg[hsp70l:bmp2b]fr13 versus WT cardiac tissue at 21.5 hpf according to Database for Annotation, Visualization and Integrated Discovery (DAVID) based clustering for functional terms (Huang et al., 2009). (B) Partial list of up-regulated cardiac genes in Tg[hsp70l:bmp2b]fr13 embryos heat-shocked at 18 hpf compared with WT. Non-muscle myosin II heavy chain subunit is marked in grey. (C-E) Unlike DMSO-treated control embryos, inhibition of NMII with Blebbistatin reduces heart tube elongation (arrows) in a dose-dependent manner. (F) Treatment with 2μM Blebbistatin has no significant (ns) effect on heart tube elongation, whereas 6.6μM Blebbistatin-treated hearts are significantly shortened at 24hpf (mean values with S.E.M.; p<0,005, **). (G) Comparison of average speed measurements for 20μM Blebbistatin-treated endocardial (green) and myocardial progenitor cells (red) reveal highly significant differences compared with WT (mean with s.d. based on quantifications of at least n=16 endocardial cells and n=16 myocardial cells per genotype; p<0.0005, ***).
Reprinted from Developmental Cell, 24(6), Veerkamp, J., Rudolph, F., Cseresnyes, Z., Priller, F., Otten, C., Renz, M., Schaefer, L., and Abdelilah-Seyfried, S., Unilateral dampening of bmp activity by nodal generates cardiac left-right asymmetry, 660-667, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell