Fig. S1

Fig. S1 Vascular defects in MZpak4 morphants. fli1a:eGFP transgenic embryos were injected with control MO (A), splice-blocking MOs (B), or the combination of the translation- (MO-3) and splice-blocking MOs (C). As indicated by the fluorescing endothelial cells, splice morphants exhibited normal vasculature (B), while defects were observed in the MZpak4 knockdown embryos (C), including short intersegmental vessels (ISV; pink arrows), abnormal longitudinal extension of ISV at myoseptum (blue arrow) and partial loss of dorsal longitudinal anastomotic vessels (DLAV). The defects could be substantially rescued by co-injection of pak4 mRNA (D). Combined results from three experiments are summarized by the histogram in (E). Images were recorded at 50 hpf.