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Fig. 7 Myocardial cells positive for pHH3 induced by H/R in vivo.
Cardiomyocytes proliferation was assessed under baseline conditions and 18 h to 30d after H/R in Tg(cmlc2:nucDsRed) zebrafish line. (a) Representative image of a zebrafish heart ventricular section 3d after H/R showing colocalization of DAPI, DsRED and pHH3 stainings. Arrows indicate cardiomyocyte pHH3+ nuclei. (b) The increase in pHH3+ cardiomyocytes was apparent 18 h after H/R, achieved its peak at the 7d time point and was back to baseline at the 30d time point (n = 3 at each time point; ** p<0.01 and *** p<0.001 vs. C).
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