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Fig. 5 Apoptotic myocyte cell death induced by H/R in vivo.
Apoptotic myocyte cell death was assessed under baseline conditions, and 18 h and 30d after H/R in the Tg(cmlc2:nucDsRed) zebrafish line. At 18 h after H/R it was found a marked increase in apoptotic myocardial cell number, which was back to control value at the 30d time point. (a) Representative image of a zebrafish heart ventricular section 18 h after H/R, showing colocalization of DAPI, DsRED and TUNEL stainings. Arrows indicate cardiomyocyte TUNEL+ nuclei, whereas arrow-head indicates non-cardiomyocyte TUNEL+ nuclei. (b) TUNEL+ cardiomyocytes nuclei in control (C) animals, and 18 h and 30d after H/R (n = 3 at each time point; * p<0.05 vs. C).
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