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Fig. 5

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ZDB-IMAGE-130220-9
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Figures for Ding et al., 2013
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Fig. 5 The viscerocranium and neurocranium of prdm5hi61Tg-prdm1am805 double mutant exhibit more severe phenotypes than single mutants. Double prdm1am805 prdm5hi61Tg heterozygotes were crossed to obtain the prdm5hi61Tg-prdm1am805 double mutant embryos. A-D: Flat-mounted Alcian blue staining of the viscerocranium and neurocranium in control, prdm1am805, prdm5hi61Tg mutant, and prdm5hi61Tg-prdm1am805 double mutant. As compared to control (A), the prdm1am805 (B) mutant larva is missing the ceratobranchial 2ā€“5 cartilages (cb2ā€“5) as was also observed in the prdm1a morphant. The prdm5hi61Tg (C) mutant larva has a slightly shortened Meckel′s cartilage (m) in the viscerocranium. The prdm5hi61Tg-prdm1am805 double mutants (D) have a more severe phenotype in the viscerocranium (shortening of the Meckel′s cartilage, greatly widening of the angle between ceratohyals [ch], and absence of ceratobranchials 2ā€“5). Eā€“H: Flat mount of the neurocranium in the control (E), prdm1am805 mutant (F), prdm5hi61Tg mutant (G), and the prdm5hi61Tg-narrowmindedm805 double mutant (H). The neurocranium and ethmoid plate (ep) are slightly smaller and trabeculae are shortened in prdm1am805 mutant and prdm5hi61Tg mutant (F,G). In prdm5-/-, the hypophyseal fenestra is significantly wider and the anterior basicapsilar cartilage is missing (G, arrow). The double mutant shows further reduction of the neurocranium, smaller ethmoid plate, shorter trabeculae, and missing basicapsilar cartilage (H, arrow). Anterior is to the left. abc, anterior basicapsular commissure; cbs, ceratobranchials; ch, ceratohyal; ep, ethmoid plate; m, Meckel′s cartilage; n, notochord; pch, parachordal; tr, trabeculae; * in B,D illustrates missing cbs.

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