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Fig. S1 Mature mRNAs are present during very early stages of oogenesis. RT-PCRs for exonic (A) and intronic (B) transcripts for β-actin and sass6 from whole ovaries day 1 post-purging (lanes 1, 2, 7, 8) and from stage IV oocytes (lanes 4, 5, 10, 11). Mature mRNAs for β-actin and sass6 isolated by oligodT priming are present in ovaries at day 1 post-purging (lanes 1, 2) and in stage IV oocytes (lanes 4, 5). Pre-spliced immature mRNAs for β-actin and sass6 isolated by random hexamer priming are also present in ovaries at day 1 post-purging (lanes 7, 8) and in stage IV oocytes (lanes 10, 11). For the negative controls (lanes 3, 6, 9 12) the respective total RNA samples was used as template in the PCR reaction with β-actin primers, to confirm that the RNA used for cDNA synthesis was free of genomic DNA.