Fig. S3

Fig. S3 Distribution of preferred directions in postsynaptic compartments in the superficial neuropil in different transgenic lines (A) Two-dimensional histogram of preferred directions in the neuropil of Tg(Oh:G-3;UAS:GCaMP3 fish. Average of normalized PD histograms from 6 individual fish (same as in Fig. 5A₆₎. Orange rectangle corresponds to upper (superficial) 35% of the neuropil, in which the DSIs were sorted by preferred directions (36 directions, bin width: 10°) and summed to construct the histogram in panel B. (B) Distribution of summed DSIs, sorted by preferred direction, and normalized. In each directional bin (rows in panel A, bin width 10°), the DSIs in the top 35% of neuropil (right area in panel A, marked by orange rectangle) were summed to calculate a relative ‘directional content’ in the postsynaptic compartments in this transgenic line. In Tg(Oh:G-3;UAS:GCaMP3), directional signals in postsynaptic compartments exhibit the highest summed DSIs for directions between 90° and 7 270°, with a peak at 130° (right arrow), a tail towards higher angles (left arrow), and a minimum between 270°-360°. This is in qualitative agreement with the distribution of PDs in somatic Ca²⁺ measurements in this line (Fig. 2C,D). (C) Same as in A, but for the Tg(Oh:G-4;UAS:GCaMP3) line. DSIs were largest for directions near the 0° (CR) direction in this line (same data as in Fig. 5B₆₎. (D) Distribution of summed DSIs for the Tg(Oh:G-4;UAS:GCaMP3) line. Same analysis as in B. Here, postsynaptic directional signals exhibit the highest summed DSIs in the 10°-bin centered around 355°, corresponding to CR-directions, consistent with pronounced CR-tuning of somatic Ca²⁺ signals in this line (Fig. 2C,D). (E) Same as in A, but for the triple transgenic Tg(Oh:G-3;Oh:G-4;UAS:GCaMP3) line. (F) Distribution of summed DSIs in the triple transgenic Tg(Oh:G-3;Oh:G-4;UAS:GCaMP3) line. Here, postsynaptic directional signals exhibit peaks at 0° (CRdirection, center arrow), at 105° (right arrow) and a small elevation at 205° (left arrow), consistent with a combination of preferred directions observed separately in Tg(Oh:G-3;UAS:GCaMP3) and Tg(Oh:G-4;UAS:GCaMP3).