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Fig. 7 Expression of fsta is increased in runx3 and egr1 morphants and sox9b mutants.
Lateral views of in situ hybridizations, anterior to the left. Scale bars 100 μm. (A-F) Compared to controls or wild-type embryos, expression of fsta is up-regulated in egr1 morphants (A,B), runx3 morphants (C,D), and homozygous sox9b mutants (E,F) at 48 hpf. pharyngeal endoderm (pe). (G,H) 4 dpf Alcian Blue stained larvae injected with MOcon 6 ng (K) and MOfsta 6ng (L). Knock-down of fsta causes a hyperplasia of the viscerocranium. Meckel’s cartilage (m), ceratohyal (ch), ceratobranchials 1 to 5 (cb1-5).
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