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Fig. 1 Expression of dcc, robo2 and robo3var2 in the zebrafish spinal cord. (A-C) Expression at 18 hpf. Adcc is expressed in the ventral half of the spinal cord. Brobo2 is expressed throughout the spinal cord and is observed in numerous postmitotic neurons (arrows). Crobo3var2 expression is restricted to postmitotic neurons at 18 hpf. D-F Expression at 24 hpf. Ddcc expression has expanded to include almost the entire spinal cord; however, expression is weaker in segmentally repeated regions in dorsal and ventral spinal cord, as indicated by arrows. Erobo2 is expressed in postmitotic neurons (arrows). F At 24 hpf, robo3var2 is expressed in postmitotic neurons. G-O Pseudocolored reflected light image of anti-GFP immunofluorescence in NBT:tau-GFP embryos (G, J, M) and transmitted light images of the same embryos that have undergone in situ hybridization for dcc, robo3var2, and robo2 (h, k, n) at 24 hpf. (i, l, o) exhibit overlain images of (G and H), (J and K), and (M and N), respectively. CoPA neurons are indicated by arrows. In all images, dorsal is up, anterior to the left. White lines indicate the dorsal and ventral boundaries of the spinal cord.