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Fig. 2 Wnt and FGF signaling play partially redundant roles in regulating NPB specifiers. (A-H) Embryos treated with SU5402 starting at 7 hpf exhibited decreased pax3a expression (100%, n=28) (B versus A), but no decrease in zic3 expression (100%, n=16) (F versus E). dkk1 overexpression induced by 7 hpf heat shock decreases expression of pax3a (100%, n=24) (C versus A) and zic3 (100%, n=16) (G versus E). When the Wnt and FGF signaling pathways are both attenuated with heat shock-induced dkk1 overexpression and SU5402 treatment starting at 7 hpf, pax3a and zic3 mRNA levels decrease more than when either pathway is knocked down alone [pax3a, D versus B,C (100%, n=24); zic3, H versus F,G (100%, n=17)]. All pictures are dorsal trunk views with anterior upwards.