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Fig. S7 Constitutively active Lef1 activates pax3a IR1, zic3 E1 and zic3 E2 activity in the absence of cycloheximide. Embryos from the pax3a IR1, zic3 E1 and zic3 E2 transgenic lines were injected with glucocorticoid receptor-tagged Lef1-β-catenin and treated with ethanol (A-C) or dexamethasone (D-F) beginning at tailbud stage. pax3a IR1 exhibited an increase in enhancer activity upon induction of Lef1-β-catinin (D versus. A) (79%, n=19) as did zic3 E1 (E versus B) (45%, n=11) and E2 (F versus C) (100%, n=11).
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