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Fig. S2 >bmp4 is not necessary for initiation or maintenance of dorsal retinal fate. bmp4 is not necessary for initiation or maintenance of dorsal retinal fate. (A-F) The dorsal-specifying genes tbx5a, gdf6a, and ephrinB2a are expressed normally in bmp4st72 mutants at 25 hpf, as is the ventral specifying gene vax2. (G-H) The ventral patterning gene vax2 is also normally expressed in bmp4 mutants at 25 hpf. mRNA expression of polarized dorsal and ventral genes (tbx2a, tbx5a, gdf6a, and vax2) is also initiated normally in bmp4st72 mutants at 12-15 hpf (data not shown). Scale Bar = 50 μm.
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Reprinted from Developmental Biology, 371(1), Kruse-Bend, R., Rosenthal, J., Quist, T.S., Veien, E.S., Fuhrmann, S., Dorsky, R.I., and Chien, C.B., Extraocular ectoderm triggers dorsal retinal fate during optic vesicle evagination in zebrafish, 57-65, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.