|
Fig. S1 In situ hybridization for selected genes following LiCl and SU5402 treatment. We assayed the expression of axin2, her1, hes6, msgn1, notum1a, sef, tbx6, and tbx24. Generally, there was no obvious change in the spatial expression domain, except for the occasional extra her1 stripe due to a shifting of the wavefront. This result had been previously reported in mice expressing a constitutively active beta-catenin in the tailbud (Aulehla et al., 2008; Dunty et al., 2008). In some cases the relative levels of expression are evident, consistent with our microarray and qPCR data. For example, msgn1 and hes6 appear slightly weaker in SU5402 treated embryos. notum1a expression is elevated in discrete domains in the tailbud and head regions following LiCl treatment and elevated specifically in the tailbud following SU5402 treatment. sef expression levels appear slightly elevated after LiCl treatment and are reduced after treatment with SU5402.
Reprinted from Developmental Biology, 369(2), Stulberg, M.J., Lin, A., Zhao, H., and Holley, S.A., Crosstalk between Fgf and Wnt signaling in the zebrafish tailbud, 298-307, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.