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Fig. 1 Glomerulus development in medaka and zebrafish pronephros in comparison to mouse and rat metanephros.
A1-B5: Pronephric glomerulus development in 3 to 6 dpf medaka (A1-A5) and 34 hpf to 4 dpf zebrafish (B1-B5) by JB4 cross sections stained by hematoxylin and eosin. The glomerular primordium of medaka exhibited a C-shaped epithelial layer (A2, A3), which is similar to the mouse and rat S-shaped body stage (C3, D3), unlike in zebrafish (B2). The C-shaped primordium contained a characteristic balloon-like or sinusoidal capillary. The paired pronephric glomerulus was fused at the midline to form a glomerulus in zebrafish (B3), but remained separated into two parts by the interposition of an interglomerular mesangium in medaka (arrowheads in A2-A5). C1-D5: Metanephric glomerulus development in rat epoxy resin sections stained by toluidine blue (C1-C5) and in mouse E18.5 kidney sections stained with H&E (D1-D5). Cross section of rat and mouse metanephros shows renal vesicle (C1, D1), comma-shaped body (C2, D2), S-shaped body at (C3, D3), capillary loop at (C4, D4), and maturing glomerulus (C5, D5). Rat vascular cleft in C2 (arrowhead) and primitive podocyte layer in C3 (asterisk). Mouse vascular cleft in D2 and D3 (arrowheads). Scale bars = 10 μm.