ZFIN Image: Avella et al., 2012, Fig. 5

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Figure Caption

Fig. 5 GnRH3 neurons soma size.

GnRH3 neurons in transgenic zebrafish larvae (n = 10) sampled at 6, 8 and 10 dpf (A). Assay was conducted in triplicate. On the right, GnRH3 neurons size (pixels) (B). The green channel of RGB images collected at magnification of 20X (whose some representative are shown here) was extracted, converted to grayscale and GnRH3:EGFP neurons were analyzed for size using the “Analyze > Analyze particles…” macro. We set up the macro-assisted algorithm to exclude the background pixelation. At 6, 8 and 10 dpf, 10 fish were analyzed for each experimental condition. Values with different superscript letters are significantly different (P<0.05).

Acknowledgments

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