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Fig. 5
Tcf/Lef-miniP:dGFP reporter was activated in the amputated caudal fin regenerative outgrowths of adult transgenic fish. The caudal fin was amputated at the white line indicated, as shown for 4 dpa (0–4 dpa). d2EGFP-expressing cells were visualized by fluorescence microscopy (lower panels). The fluorescence images were merged with bright-field (BF) images (upper panels). The Tcf/Lef-miniP:dGFP reporter was upregulated in the amputated fin during regeneration. Scale bar, 200 μm.
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Reprinted from Developmental Biology, 370(1), Shimizu, N., Kawakami, K., and Ishitani, T., Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/beta-catenin signaling-reporter transgenic zebrafish, 71-85, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.