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Fig. 7
Expression of saMSTN-2 gene in brain and muscle of S. aurata fish. Total RNA from four brains (#8G, 26G, 23C and 33C) and four muscle samples (#1G, 1F, 3F and 3S) was reverse-transcribed using oligo(dT) and subjected to PCR amplification of MSTN-2. β-actin was used as a control for absence of genomic DNA in the RNA samples. Genomic DNA from four fish was amplified using the same primers for MSTN-2 and β-actin. Amplified products were analyzed on 1-1.5% agarose gels and photographed under UV illumination following staining with ethidium bromide.
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Reprinted from Molecular and Cellular Endocrinology, 361(1-2), Nadjar-Boger, E., Hinits, Y., and Funkenstein, B., Structural and functional analysis of myostatin-2 promoter alleles from the marine fish Sparus aurata: Evidence for strong muscle-specific promoter activity and post-transcriptional regulation, 51-68, Copyright (2012) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.