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S2 Influence of mounting conditions on morphological development. Embryos were embedded in 1.5% agarose, 0.1% agarose in an FEP tube, or 3.0% methylcellulose in an FEP tube at 24 hpf and imaged in transmission with SPIM. Images were taken every 6 hours. The embryo embedded in 1.5% agarose is unable to undergo morphological changes and died at 50 hpf. Multilayer mounting methods allow outgrowth of the head.
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