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Fig. 3

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ZDB-IMAGE-121004-19
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Figures for An et al., 2012
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Fig. 3 Molecular consequences of sf3b1 mutations (A,B).Variant transcripts of sf3b1 in tstb460 mutants. (A) RT-PCR products were amplified between exon1 and exon6 of sf3b1 with 20-24 hpf AB wildtype and tstb460 homozygous mutant total RNA as template using Sf3b1aa (46)F and Sf3b1aa (579)R primers. The single AB wildtype RT-PCR product was 534 bp (tran1). tstb460 homozygous mutants contained 4 different transcripts; tran1(534 bp), and variant transcripts tran2(412 bp), tran3(390 bp), and tran4(312 bp). (B) Diagram of variant transcripts in tstb460 homozygous mutants. (C) Western blot analysis of Sf3b1 protein levels in wildtype, tstb460 mutant and hi3394a mutant embryos at 24 hpf. Lane 1: wildtype embryos; Lane 2: tstb460 homozygous mutants; Lane 3: hi3394a homozygous mutants; Lane 4: hsp>sf3b1 injected tstb460 mutants with melanophore phenotype rescue.

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