ZFIN Image: Cunningham et al., 2012, Fig. 1

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Figure Caption

Fig. 1 NSC140873, Ro5-3335, and Ro24-7429 inhibit definitive hematopoiesis in zebrafish embryos. (A) NSC140873 reduced circulating cd41-GFP⁺ (Upper) and lck-GFP⁺ (Lower) cells in transgenic zebrafish embryos compared with DMSO-treated embryos. Red arrows point to circulating cd41-GFP⁺ cells in the heart and lck-GFP⁺ cells in the thymus, respectively. (B) Dose-dependent reduction of circulating cd41-GFP cells by NSC140873. (C) Chemical structures of the three related compounds. (D) Dose-dependent reduction of circulating cd41-GFP cells by Ro5-3335 and Ro24-7429. (E) The runx1 W84× truncation mutation sensitizes fish embryos to the inhibition by Ro5-3335. *Numbers of untreated runx1^+/- embryos or DMSO-treated runx1^+/- embryos with intermediate or no visible circulating CD41-EGFP⁺ cells were compared. ^#Numbers of runx1^+/- embryos or wild-type embryos with intermediate or no visible circulating CD41-EGFP⁺ cells after Ro5-3335 treatments were compared. For all comparisons in B, D, and E, the P values were calculated with 2 × 2 contingency tables and Fisher’s exact test.

Acknowledgments

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