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Fig. S2 Transmission electron microscopy shows that the trunk region of the dorsal aorta of the zebrafish embryo was devoid of smooth muscle cells. This image, taken from the midpoint of the region of the aorta used for time based imaging, demonstrates that no smooth muscle cells are present around the vessel at 5 dpf. Skeletal muscle cells are clearly visible, as is the pronephros (PN). Erythrocytes (RBC) are abundant within the vessel, and endothelial cells are clearly evident. No smooth muscle cells can be detected. (Scale Bar = 20 microns).
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