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Fig. 5
Rescue of hindbrain upon an all-trans RA pulse at various developmental stages. Rescue of hindbrain development in zebrafish embryos expressing GFP in r3 and r5, continuously incubated in 10 μM DEAB from sphere stage (4 hpf) and exposed for 5 minutes to 1 nM all-trans RA (tRA) at various developmental stages (except for the control incubated in embryo medium, EM). On the left are shown typical patterns of GFP expression at 24 hpf. On the right is shown the average ratio of total fluorescence in r5 versus r3. Note that exposure to all-trans RA until bud stage yields an almost normal pattern of hindbrain development, whereas exposure to all-trans RA at the 3-somite stage (11 hpf) does not rescue normal development of r5. Error bars indicate s.e.m. Scale bar: 200 μm.