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Fig. 2

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ZDB-IMAGE-120830-3
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Figures for Yan et al., 2012
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Fig. 2

Morphological phenotypes of zip7 loss with MO microinjection and/or zip7 mRNA rescue at 72 hpf embryos.

(A) Fluorescent images of live 24 hpf, GFP-Reporter control embryos (n = 57/60) and GFP-Reporter+zip7-MO morphant embryos (n = 62/62) indicate inhibition of zip7-GFP reporter expression (green, white arrows) by zip7-MO. (B–D) Lateral views (anterior to the left) of embryos at 72 hpf (B) Wild type with control-MO microinjection (2 nL, 10 ng). (C) Wild type with zip7-MO microinjection (2 nL, 10 ng). (D) Wild type with zip7-MO (1 nL, 10 ng) + zip7 mRNA (bottom, 1 nL, 225 pg) microinjection. (E) Statistical analysis of microinjection of zip7-MO and zip7 mRNA phenotypes.

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