ZFIN Image: Schoenebeck et al., 2012, Fig. S5

Image

Figure Caption

Fig. S5 Y/F→L substitutions differentially affect Tgfßs. (A–C,E–G) Whole mount embryos at embryonic stage 24 hpf (A–C) or 28 hpf (E–G), anterior to the left. Phenotypes are representative of (A,E) normal, (B) dorsalized, (C) mildly dorsalized, (F) ventralized, (G) mildly ventralized classes following injections. (A–D) Embryos injected with mouse Gdf1or Gdf1^F→L mRNA. (E–H) Embryos injected with either zebrafish bmp2b or bmp2b^Y→L mRNA. (D,H) Stacked bar graphs depicting frequency of observed phenotypes. Number of embryos injected per mRNA concentration appears above columns. While a missense mutation strongly reduces GDF1 dorsalizing activity, a comparable mutation in Bmp2b has little affect on this molecule′s ventralizing activity.

Acknowledgments

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