Fig. 2

Fig. 2  ovl/ift88 Mutant Hair Cells Lack Kinocilia yet Develop Normally(A and B) SEM image of an ift88^tz288b immature (A) and mature (B) hair bundle.(C and D) Tubulin and actin stain of bundles at 5 dpf in wild-type (WT) (C) and ift88^tz288b (D).(E) The average number of hair cells per neuromast at 2 dpf in wild-type and ift88^tz288b neuromasts (n > 13 neuromasts). ns, not significant.(F) Proportion of FM 1-43-labeled hair cells in ift88^tz288b and wild-type neuromasts at 2 dpf (n > 13 neuromasts).(G) The average FM 1-43 intensity per neuromast at 2 dpf (n > 13 neuromasts). a.u., arbitrary units.An unpaired Student’s t test was used to compare number of hair cells per neuromast in (E) and (G). A chi-square Fisher’s exact test was used to compare proportion of FM 1-43-labeled hair cells in (F). Scale bars, 500 nm (A and B) and 5 μm (C and D). Error bars are SEM.