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Figure Caption
Fig. S6 The antioxidant N-acetyl-L-cysteine can alleviate the blood phenotype of 4-oxo-4HPR-treated zebrafish embryos. 2 dpf zebrafish (n=35 per condition) raised in the presence of PTU were manually de-chorionized and treated with 1 7mu;M 4-oxo-4HPR alone or in the presence of 5 mM N-acetyl-l-cysteine (NAC). Vehicle only-treated larvae (WT/Vehicle) served as controls. Upon treatment, 3 dpf (upper panels) and 4 dpf (lower panel) fish were collected and stained for hemoglobin using o-Dianisidine as outlined in the Materials and methods.
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