Fig. 3

Fig. 3 The sqt 3′UTR is necessary and sufficient for dorsal activity of sqt RNA. (A) Schematic of constructs used to express mutant sqt fused with globin 3′UTR (black; sqt^STOP:glo), sqt 32UTR (blue; sqt^STOP:sqt), venus (green) fused with globin 3′UTR (venus:glo) or sqt 3′UTR (venus:sqt). (B-I) Expression of gsc at 30% epiboly (B-E) is expanded in zebrafish embryos injected with sqt^STOP:sqt (C) or venus:sqt (E), but not with sqt^STOP:glo (B) or venus:glo (D). Dorsal expansion by the sqt 3′UTR is transient and is not detected at 60% epiboly (F-I). (J) Angle of gsc expansion in injected embryos at 30% epiboly. Each blue dot represents a single embryo. N indicates the number of injected embryos for each RNA and μ(°) shows mean gsc angles. Scale bar: 100 μm.