ZFIN Image: Lim et al., 2012, Fig. S4

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Figure Caption

Fig. S4 sqtMO1 does not affect the stability of sqt RNA. (A) Semi-quantitative RT-PCR analysis of endogenous sqt RNA in uninjected, Con MO-injected or sqtMO1-injected embryos at the 8-cell stage shows that sqt RNA levels remain uniform. (B) Quantitative real-time RT-PCR to detect endogenous sqt RNA in uninjected embryos, embryos injected with Con MO and embryos injected with sqtMO1 shows that sqt RNA levels remain unchanged at least until the 8-cell stage. Bar chart shows the relative expression of sqt RNA, normalized against actin RNA levels. The experiment was performed three times; error bars indicate the s.d. between independent experiments.

Acknowledgments

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