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Fig. S5 Overexpression of dnPKA does not affect sox17-positive endodermal cell numbers. (A-D) In situ hybridisation for sox17 at 9 hpf in control embryos (A,B) and embryos injected with 200 pg dnPKA mRNA (C,D). (A,C) Dorsal views; (B,D) animal pole views. Although injection of ptc MOs gave an increase in sox17-positive endodermal cells at 9 hpf, no significant increase in endodermal cells was detected in embryos injected with 200 pg dnPKA mRNA (compare D with Fig. 1E), consistent with the lack of increased proliferation in dnPKA-injected endodermal donor cells (see Fig. 6A). (E) Injection of higher amounts of dnPKA mRNA (300 pg and 400 pg) did not significantly change proliferation of endodermal donor cells in the transplant assay, suggesting that the lack of significant effects of dnPKA mRNA injection on endodermal cell proliferation is not due to insufficient amounts of dnPKA.