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Fig. 3 Cxcr4a is required for endodermal cell proliferation and determines the proliferative response to Hh signalling. (A,B) In situ hybridisation for sox17 at 9 hpf in control (A) and cxcr4a morphant (B) zebrafish embryos. (C) Quantification of pHH3 and Tg(sox17:EGFP) double-positive endoderm cells in cxcr4a single-morphant and smo;cxcr4a double-morphant Tg(sox17:EGFP) embryos. Bars show mean percentage of double-positive cells; error bars indicate s.e.m. n=18 (control), n=23 (cxcr4a MO) and n=31 (smo;cxcr4a double MO) embryos in three independent experiments. (D,E) Quantification of transplant assay results using endodermal (D) and non-endodermal (E) donor cells. Bars show mean fold change of three independent experiments. n values are given in supplementary material Table S2. *P<0.05; **, P<0.01; ***P<0.001; Student′s t-test (C) or one-way ANOVA analysis (D,E).