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Fig. S2 A proliferation assay based on cell transplantation. Host embryos with donor cells at 6 hpf (A,D) or 10 hpf (B,C,E,F). (A-C) Injection of cas mRNA converts donor cells into endoderm, as indicated by Tg(sox17:EGFP) expression at 10 hpf (C). Injection of smo MO blocks proliferation of endodermal donor cells (A,B). (D-F) Cells from donor embryos that were not injected with cas mRNA do not express Tg(sox17:EGFP) (F), indicating that they become mesodermal and/or ectodermal.
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