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Fig. S3 Hh signalling regulates p57 expression in gastrula stage zebrafish embryos. In situ hybridisation for p57 at 9-10 hpf in control (A), cas (B), smo (C) and ptc (D) morphant embryos. (A) High levels of p57 expression were detected in axial mesoderm and additional cell populations. (B) This expression pattern is not changed in cas morphant embryos, which lack endoderm, suggesting that p57-expressing cells are mesodermal and/or ectodermal. (C) In smo morphants, axial expression of p57 persists, although p57 expression is abolished in other domains of the embryo. (D) In ptc morphants, the expression of p57 is expanded.