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Fig. S2 ihha is exclusively expressed along the vp edge and colocalizes with sp7:EGFP expression. Confocal projections of whole-mount fluorescent in situ hybridization at 5 dpf. (A) The sp7:EGFP transgene is expressed highly along the vp edge at 5 dpf. (B) Endogenous sp7 expression colocalizes with transgene expression with high levels seen specifically along the vp edge at 5 dpf. (C) Confocal projection of an image with overexposed vp edge expression to show sp7 expression at lower levels throughout the entire Op. (D) Overexposed image of ihha expression reveals exclusive expression along the vp edge.