ZFIN Image: Huang et al., 2012, Fig. S2

Image

Figure Caption

Fig. S2 LFP cells can be reliably identified based on their locations. (A) Gata2-GFP embryos were stained with the shh probe (green), the GFP antibody (red) and the Draq5 dye (blue) to label cell nuclei. Gata2-GFP-positive KA′′ cells (short arrows) and Gata2-GFP-negative LFP cells (long arrows) flank the shh-expressing medial floor plate cells (arrowheads). (B) Gata2-GFP embryos were stained with the tal2 probe (green), the GFP antibody (red) and the Draq5 dye (blue). All Gata2-GFP-positive KA′′ cells (short arrows) also express tal2. (C) nkx2.2a-mEGFP embryos were stained with the tal2 probe (green), the GFP antibody (red) and the Draq5 dye (blue). All tal2-negative cells (long arrows) immediately flanking the medial floor plate (arrowheads) are LFP cells, indicated by the expression of membrane localized EGFP under the control of the nkx2.2a promoter (nkx2.2a-mEGFP). Dorsal views of embryos at 21 hpf are shown. Scale bars: 20 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.