ZFIN Image: Moro et al., 2012, Fig. 7

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Figure Caption

Fig. 7

Persistent TCFsiam expression allows fine tracking of neural crest-derived cells. (A) sox10:mRFP expressing cells in otic vesicle of a representative 36 hpf double transgenic larva Tg(sox10:mRFP)^vu234/(7xTCF-Xla.Siam:GFP)^ia4 coexpress the Tg(7xTCF-Xla.Siam:GFP)^ia4 transgene. (B,C) Neural crest-derived cells in the pharyngeal arches coexpress the transgene Tg(7xTCF-Xla.Siam:GFP)^ia4 at a high level at 36 hpf. (D) At 72 hpf, Tg(sox10:mRFP)^vu234/Tg(7xTCF-Xla.Siam:GFP)^ia4 coexpressing cells are dorsal root ganglia (DRG) scattered along the trunk. (E) Montage of a time lapse movie, showing neural crest-derived cells dynamics in the midbrain of a representative 56 hpf Tg(sox10:mRFP)^vu234/Tg(7xTCF-Xla.Siam:GFP)^ia4 larva. One to two small white arrows in the bottom left indicate migration and neural crest derived cell division along a brain vessel. Note at the 13th frame a small inset showing a doublet of sox10 positive cells coexpressing the TCFsiam transgene. Each frame was recorded every 30 min. (F) Sequential images of a time lapse movie in a representative 5 dpf Tg(sox10:mRFP)^vu234/Tg(7xTCF-Xla.Siam:GFP)^ia4 larva showing the migration of double positive cells across the somitic mesoderm. Copositive migrating cells are labeled by a white arrow. Each frame was recorded every 1 h.

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Reprinted from Developmental Biology, 366(2), Moro, E., Özhan, G., Mongera, A., Beis, D., Wierzbicki, C., Young, R.M., Bournele, D., Domenichini, A., Valdivia, L.E., Lum, L., Chen, C., Amatruda, J.F., Tiso, N., Weidinger, G., and Argenton, F., In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains, 327-340, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.