ZFIN Image: Moro et al., 2012, Fig. 5

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Figure Caption

Fig. 5

Expression analysis of the TCFsiam transgene in wild-type AV canal between 48 and 96 hpf. (A, B, D, E) Confocal images of the AV canal from Tg(myl7:EGFP)^twu34/Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5 fish (A, D, E) and from Tg(kdrl::EGFP)^s843/Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5 double transgenics (B,C,G,H,I). (F,I) are cross section from the AV canal of Tg(Tie2:EGFP)^s849/Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5 (F) and Tg(kdrl:EGFP)^s843/Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5 fish (I). (C) Cross section of the bulbus arteriosus (BA) of a 96 hpf Tg(kdrl:EGFP) ^s843/Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5 fish. Green fluorescence has been pseudocolored in blue for Tg(myl7:EGFP) ^twu34 in D, E, for Tg(kdrl::EGFP) ^s843 in C,G,H,I and for Tg(Tie2:EGFP) ^s849 in F. (A–I). Immunostaining with zn8 (blue in A, pseudocolored green in C–I). (A) Few atrial myocardial cells adjacent to the atrioventricular canal (arrowhead) express the Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5. (B) Endocardial cells forming the multilayered structure of cardiac valves at 72 hpf express the transgene. (C) Endocardial cells at the boundary between the ventricle and bulbus arteriosus express the transgene (arrowheads). (D, E, G, H, I) round shaped cells at the AV canal adjacent to the cuboidal epithelial cells express the transgene. (D–I) Tg(7xTCF-Xla.Siam:nlsmCherry)^ia5 positive cells downregulate alcam/zn8 at the AV boundary and the AV marker Tg(Tie2:EGFP) ^s849 (F). A:atrium, V:ventricle; AV: atrio-ventricular.

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Reprinted from Developmental Biology, 366(2), Moro, E., Özhan, G., Mongera, A., Beis, D., Wierzbicki, C., Young, R.M., Bournele, D., Domenichini, A., Valdivia, L.E., Lum, L., Chen, C., Amatruda, J.F., Tiso, N., Weidinger, G., and Argenton, F., In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains, 327-340, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.