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Fig. S3
An internal 400bp promoter is sufficient to drive specific transgene expression in the dorsal EVL and dorsal periderm. A single embryo (A) and a group of 8 embryos (B) at 40% epibody show GFP expression in the presumptive dorsal site. At 24hpf, the larva shows GFP expression in the dorsal periderm (C). Transient expression analysis revealed that 10% of the injected embryos (N = 144) show specific GFP expression in the dorsal EVL and dorsal periderm.
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Reprinted from Mechanisms of Development, 129(1-4), Chen, Y.Y., Harris, M.P., Levesque, M.P., Nüsslein-Volhard, C., and Sonawane, M., Heterogeneity across the dorso-ventral axis in zebrafish EVL is regulated by a novel module consisting of sox, snail1a and max genes, 13-23, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.