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Fig. 6 Detection of the ectopic expression of myrAkt1 transgene in Tg(krt4:Hsa.myrAkt1)cy18.
(A) Schematic diagram showing the chromosomal integration site in Tg. (B) Relative expression level of myrAkt1 transgene among different tissues assayed by real-time RT-PCR. Stars above the error bars indicate significant differences as tested by Student’s t-test (**p<0.01, and ***p<0.001). The detection of the expression of a Akt downstream target of phospho-mTOR in the adipose tissues of WT (C) and Tg (D) by immunohistochemistry. WT, wild type; Tg, Tg (krt4:Hsa.myrAkt1)cy18.
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