ZFIN Image: Royo et al., 2011, Fig. S3

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Figure Caption

Fig. S3

Controls suggest a low enhancer trapping capacity of the empty ZED vector. Diagram showing the structure of the ZED vector (A). Transgenic zebrafish evaluated at 48 hpf from six independent founders obtained from the ZED-zero construct. Pictures evidenced both spurious or no EGFP expression (B–F), despite strong RFP expression in the somites (G). Abbreviations: Tol2: Tol2 transposase target site; C. Actin: cardiac actin promoter; rfp; red fruorescent protein gene; ins: insulator; gfp: green fluorescent protein gene; Min. Prom: minimal promoter; entry site: gateway entry site, which was eliminated to generate the ZED-zero construct.

Acknowledgments

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